The Knowledge Translation (KT) for Zero-Dose Immunization Research toolkit is a guide for effectively transforming research findings into practical applications, ensuring that evidence-based knowledge is communicated, adopted, and used. Designed by the Zero-Dose Learning Hub for Country Learning Hubs in Bangladesh, Mali, Nigeria, and Uganda, this toolkit provides a step-by-step process for developing KT plans. It includes guiding questions, links to resources, and strategies for engaging stakeholders, particularly in zero-dose immunization research. The toolkit aims to close the know-do gap, promote evidence-based practices, and drive impactful changes in policies and practices.